MERS coronavirus envelope protein has a single transmembrane domain that forms pentameric ion channels
Identifieur interne : 001711 ( Main/Exploration ); précédent : 001710; suivant : 001712MERS coronavirus envelope protein has a single transmembrane domain that forms pentameric ion channels
Auteurs : Wahyu Surya [Singapour] ; Yan Li [Singapour] ; Carmina Verdià-Bàguena [Espagne] ; Vicente M. Aguilella [Espagne] ; Jaume Torres [Singapour]Source :
- Virus Research [ 0168-1702 ] ; 2015.
Descripteurs français
- KwdFr :
- Canaux ioniques (), Canaux ioniques (métabolisme), Conformation des protéines, Coronavirus du syndrome respiratoire du Moyen-Orient (), Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie), Humains, Multimérisation de protéines, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (métabolisme).
- MESH :
- métabolisme : Canaux ioniques, Protéines de l'enveloppe virale.
- physiologie : Coronavirus du syndrome respiratoire du Moyen-Orient.
- Canaux ioniques, Conformation des protéines, Coronavirus du syndrome respiratoire du Moyen-Orient, Humains, Multimérisation de protéines, Protéines de l'enveloppe virale.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Ion Channels, Viral Envelope Proteins.
- chemical , metabolism : Ion Channels, Viral Envelope Proteins.
- chemistry : Middle East Respiratory Syndrome Coronavirus.
- physiology : Middle East Respiratory Syndrome Coronavirus.
- Humans, Protein Conformation, Protein Multimerization.
Abstract
The envelope protein of MERS coronavirus (MERS-CoV E protein) has been purified. MERS-CoV E protein forms pentameric ion channels. MERS-CoV E protein has one transmembrane domain. The full length construct obtained is amenable to structural determination by NMR in detergents.
Url:
DOI: 10.1016/j.virusres.2015.02.023
PubMed: 25733052
PubMed Central: 7114494
Affiliations:
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Aguilella</name><affiliation wicri:level="2"><nlm:aff id="aff0010">Department of Physics, Laboratory of Molecular Biophysics, Universitat Jaume I, 12071 Castellón, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Physics, Laboratory of Molecular Biophysics, Universitat Jaume I, 12071 Castellón</wicri:regionArea><placeName><region nuts="2" type="communauté">Communauté valencienne</region></placeName></affiliation></author><author><name sortKey="Torres, Jaume" sort="Torres, Jaume" uniqKey="Torres J" first="Jaume" last="Torres">Jaume Torres</name><affiliation wicri:level="1"><nlm:aff id="aff0005">School of Biological Sciences, Nanyang Technological University, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>School of Biological Sciences, Nanyang Technological University</wicri:regionArea><wicri:noRegion>Nanyang Technological University</wicri:noRegion></affiliation></author></analytic><series><title level="j">Virus Research</title><idno type="ISSN">0168-1702</idno><idno type="eISSN">1872-7492</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans</term><term>Ion Channels (chemistry)</term><term>Ion Channels (metabolism)</term><term>Middle East Respiratory Syndrome Coronavirus (chemistry)</term><term>Middle East Respiratory Syndrome Coronavirus (physiology)</term><term>Protein Conformation</term><term>Protein Multimerization</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Canaux ioniques ()</term><term>Canaux ioniques (métabolisme)</term><term>Conformation des protéines</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (physiologie)</term><term>Humains</term><term>Multimérisation de protéines</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Ion Channels</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ion Channels</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Canaux ioniques</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Protein Conformation</term><term>Protein Multimerization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Canaux ioniques</term><term>Conformation des protéines</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term><term>Humains</term><term>Multimérisation de protéines</term><term>Protéines de l'enveloppe virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Highlights</title><p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label><p id="par0005">The envelope protein of MERS coronavirus (MERS-CoV E protein) has been purified.</p></list-item><list-item id="lsti0010"><label>•</label><p id="par0010">MERS-CoV E protein forms pentameric ion channels.</p></list-item><list-item id="lsti0030"><label>•</label><p id="par0140">MERS-CoV E protein has one transmembrane domain.</p></list-item><list-item id="lsti0025"><label>•</label><p id="par0025">The full length construct obtained is amenable to structural determination by NMR in detergents.</p></list-item></list></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Almazan, F" uniqKey="Almazan F">F. 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Perlman</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Espagne</li><li>Singapour</li></country><region><li>Communauté valencienne</li></region></list><tree><country name="Singapour"><noRegion><name sortKey="Surya, Wahyu" sort="Surya, Wahyu" uniqKey="Surya W" first="Wahyu" last="Surya">Wahyu Surya</name></noRegion><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><name sortKey="Torres, Jaume" sort="Torres, Jaume" uniqKey="Torres J" first="Jaume" last="Torres">Jaume Torres</name></country><country name="Espagne"><region name="Communauté valencienne"><name sortKey="Verdia Baguena, Carmina" sort="Verdia Baguena, Carmina" uniqKey="Verdia Baguena C" first="Carmina" last="Verdià-Bàguena">Carmina Verdià-Bàguena</name></region><name sortKey="Aguilella, Vicente M" sort="Aguilella, Vicente M" uniqKey="Aguilella V" first="Vicente M." last="Aguilella">Vicente M. 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